Labroots

CRISPR Screening Made Easy with the Revvity Dharmacon™ All-in-One Lentiviral Platform

Revvity's Dharmacon All-in-one lentiviral platform has expanded to include whole-genome library options for CRISPR knockout (CRISPRko), CRISPR interference (CRISPRi), and CRISPR activation (CRISPRa).
Miami University

A06 - Tool development for the use of CRISPR to knockout TAT genes in cyanobacteria

Design a CRISPR construct to knockout TAT genes in cyanobacteria. Identify the optimal CRISPR plasmid backbone, design and insert the guide RNA (gRNA) into the plasmid, synthesize and insert repair ...
EurekAlert!

Cellular regulator of mRNA vaccine revealed... offering new therapeutic options

Genome-wide CRISPR knockout screens to identify cellular regulators of LNP-based mRNA vaccines. mRNAs encoding Green Fluorescent Protein (GFP) were in vitro transcribed (IVT) and delivered to knockout ...
Labroots

Stricter Precision: Enhanced Dual-Level Control of Inducible Cas9 Systems for Regulating Functional Gene Expression w/ Live Q&A

CRISPR-Cas9 has been widely adapted for use in transcriptional modulation and epigenetic engineering with deactivated Cas9 (dCas9) systems to enable CRISPR interference (CRISPRi) and CRISPR activation ...