Even those who maintain that super-resolution microscopy is a powerful tool of biological discovery have admitted that it may have a bit of an image problem. For example, in a recent review, several ...

Example of super-resolution microscopy: The image shows how the Discrete Molecular Imaging (DMI) technology visualizes densely packed individual targets that are just 5 nanometer apart from each other ...

To study living organisms at ever smaller length scales, scientists must devise new techniques to overcome the so-called diffraction limit. This is the intrinsic limitation on a microscope's ability ...

To unravel the complexities of biological phenomena, scientists have long relied on microscopy to visualize the intricate details of their specimens, including tissue architecture, cell morphology, ...

eDL-cSIM: An AI-driven super-resolution imaging method that captures high-quality live-cell dynamics in a single exposure, enhancing speed, resolution, and environmental robustness for advanced ...

With the rapid revolution in super-resolution microscopy, the resolution of far-field optical microscopy has entered the sub-nanometer era, providing new insights into macromolecules in vitro and in ...

A decade ago, the Nobel Prize in Chemistry was awarded to a trio of researchers for the development of super-resolved fluorescence microscopy. The announcement at the time stated that the researchers’ ...

What is Photoactivated Localization Microscopy? Photoactivated Localization Microscopy (PALM) is a super-resolution imaging technique that allows for the visualization of biological structures at the ...

Researchers at Istituto Italiano di Tecnologia (IIT-Italian Institute of Technology) have developed an innovative microscopy technique capable of improving the observation of living cells. The study, ...

Fluorescence images of nuclei in a cell spheroid labeled by rsGamillus-S with a diameter of 100 µm observed with widefield, SPA-SIM, and 3DSIM at a depth of 43 µm. Credit: From Nature Methods (2024).